Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Biomol Screen. 2011 Feb 18;16(4):383–393. doi: 10.1177/1087057110397357

Fig. 3.

Fig. 3

CID1340132 increases apoptosis in cells expressing mutant PIK3CA and in PTEN−/− cells. (A) isogenic HCT116 cells expressing mutant PIK3CA (PIK3CA Mut) or wild-type PI3KCA (PIK3CA WT) were treated with the indicated concentrations of CID1340132 for 48 h. cells were harvested, fixed with 70% ethanol, and labeled with propidium iodide. The sub-g1 fraction was determined by flow cytometric analysis. (B) isogenic PTEN+/+ or PTEN−/− Hec1A cells were treated with the indicated concentrations of CID1340132 for 72 h and analyzed as in a. (C) Lysates from PIK3CA Mut or PIK3CA Wt cells treated with the indicated concentrations of CID1340132 for 48 h were harvested and subjected to immunoblot analysis with the indicated antibodies. (D) Lysates from PTEN+/+ or PTEN−/− Hec1A cells were treated with the indicated concentrations of CID1340132 for 72 h and were harvested and subjected to immunoblot analysis with the indicated antibodies. PARP, poly(ADP-ribose)polymerase.