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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Biomol Screen. 2011 Feb 18;16(4):383–393. doi: 10.1177/1087057110397357

Fig. 5.

Fig. 5

CID1340132 induces DNA damage in isogenic PIK3CA mutant HCT116 cells. (A) Lysates from PIK3CA Mut or PIK3CA WT cells treated with the indicated concentrations of CID1340132 for 48 h were harvested and subjected to immunoblot analysis with the indicated antibodies. Results from three independent experiments are shown. (B) PIK3CA Mut or PIK3CA WT cells growing on cover glass were treated with DMSO or 20 μM CID1340132 for 30 h and were fixed with cold methanol and immunostained with antibody specific for γ-H2aX. PIK3CA WT cells treated with 10 μg/mL etoposide for 8 h were used as positive control. γ-H2aX foci were observed by confocal microscopy. representative images from three independent experiments are shown. (C) PIK3CA Mut or PIK3CA WT cells were treated with DMSO or CID1340132 at the indicated concentration. After incubation for 30 h, cultures were washed with phosphate-buffered saline, and attached cells were harvested and analyzed by the Oxiselect comet assay Kit as described in Materials and Methods. Representative electrophoresis images from three independent experiments are shown. Arrows indicate damaged nuclei. **p < 0.01. ***p < 0.001.