Table 2.
Effect of the TSA-dependent epigenomic modulation of MSCs on the in vitro developmental outcome of cloned pig embryos.
| TSA-mediated epigenetic transformation of MSCs | Number of oocytes/embryos | Development to | |||
|---|---|---|---|---|---|
| Enucleated | Electrofused (%) | Cleaved (%) | Morulae (%) | Blastocysts (%) | |
| + | 186 | 178/186 (95.7) | 174/178 (97.8) | 155/178 (87.1)a | 116/178 (65.2)A |
| − | 293 | 275/293 (93.9) | 262/275 (95.3) | 216/275 (78.5)b | 125/275 (45.5)B |
TSA: trichostatin A; MSC: mesenchymal stem cell.
Values with different small superscript letters (a and b) within the same column denote statistically significant differences between experimental groups (P < 0.05; χ 2 test). Values with different large superscript letters (A and B) within the same column denote very highly significant differences (P < 0.001; χ 2 test). Number of replicates ≥6.