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. 2014 Nov 6;6(1):43–55. doi: 10.18632/oncotarget.2723

Figure 1. Syringaresinol promotes cell survival and reduces cell damage after H/R in myocardial H9c2 cells.

Figure 1

(A) Chemical structure of syringaresinol. (B) Cytotoxicity of syringaresinol was tested by treating H9c2 cells with the indicated concentration of syringaresinol for 24 h. (C) Experimental design. To mimic the ischemic injury in vitro, H9c2 cells were incubated in a medium without glucose and serum for 16 h of hypoxia followed by reoxygenation in a medium with 10% FBS for 9 h. Syringaresinol was added to the medium at the beginning of the reoxygenation phase. Ctrl:control; H/R: hypoxia/reoxygenation; SYR: syringaresinol. (D) H9c2 cells were exposed to hypoxia for 16 h and treated with different concentrations of syringaresinol at the beginning of reoxygenation. After 9 h of reoxygenation, cell viability was determined by MTT assay. (E) The release of LDH in culture medium was determined at the end of reoxygenation. Results were expressed as percentages of control and presented as mean ± SD for six independent experiments. #P < 0.001 versus control group, *P < 0.001 versus H/R treatment group.