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. 2014 Nov 6;6(1):43–55. doi: 10.18632/oncotarget.2723

Figure 3. Syringaresinol promotes the degradation of HIF-1 and blocks the upregulation of a HIF-1α target gene, Bnip3 during H/R.

Figure 3

H9c2 cells were exposed to hypoxia stress for 16 h followed by treatment with 25 μM syringaresinol and reoxygenation for a further 9 h. 25 μM MG132 was added for the final 4 h. (A) Expression of HIF-1α protein was determined by Western blot. (B) Quantification of HIF-1α protein levels over actin as loading control. (C) HIF-1α mRNA levels were determined by real-time RT-PCR. The levels were quantified and normalized using GAPDH. (D) Expression of BNIP3 protein was determined by Western blot. (E) Quantification of BNIP3 protein levels over actin as loading control. All results are representative or means ± SD of six independent experiments. #P < 0.001 versus control group, *P < 0.001 versus H/R treatment group. Ctrl:control; H/R: hypoxia/reoxygenation; SYR: syringaresinol.