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. 2014 Nov 6;6(1):43–55. doi: 10.18632/oncotarget.2723

Figure 5. Syringaresinol-induced destabilization of HIF-1α and protection against cell death is mediated by FOXO3.

Figure 5

H9c2 cells were transfected with control, SIRT1 or FOXO3 siRNA. After 24hr, cells were exposed to H/R in the presence and absence of syringaresinol. (A) At 48 h after transfection of siRNAs, suppression of SIRT1 and FOXO3 expression was validated by Western blot analysis. (B) The expression levels of HIF-1α protein. (C) Quantification of HIF-1α protein levels over actin. (D) Cell death in the cultures was determined by flow cytometry. (E) Quantification of the percentages of early (annexin V-FITC positive and PI negative) and late (annexin V-FITC positive and PI positive) apoptotic cells. All results are representative or means ± SD of six independent experiments. #P < 0.001 versus control group, *P < 0.001 versus H/R treatment group. Ctrl:control; H/R: hypoxia/reoxygenation; SYR: syringaresinol.