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. 2014 Nov 25;6(1):56–70. doi: 10.18632/oncotarget.2695

Figure 3. Epigenetic priming does not potentiate the effects chemotherapy on colony growth.

Figure 3

H358 and A549 cells were seeded on a solidified Matrigel layer six days after epigenetic therapy. Beginning the following day, cells were treated with chemotherapy for 72 hours. Drug was then removed and colonies were permitted to grow 2 – 4 additional days. (A) Representative H358 colonies following treatment with 10 nM gemcitabine or 10 nM 17-AAG. (B) H358 percent colony formation (+/− standard deviation) relative to untreated control (PBS or DMSO), calculated from one representative experiment with five replicates. (C) Representative A549 colonies following treatment with 600 nM cisplatin, 30 nM 17-AAG, or 1 nM docetaxel. (D) A549 percent colony formation (+/− standard deviation) relative to untreated control (PBS or DMSO), averaged from two independent experiments (total nine replicates). Statistical significance by ANOVA with Tukey's multiple comparison test denoted as follows: *p < 0.05, **p < 0.01.