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. 2014 Nov 6;6(1):71–84. doi: 10.18632/oncotarget.2698

Figure 1. Cancer stem cell characterization.

Figure 1

A. Flow cytometry analysis of CD44 and ALDH activity. The CD44+/ALDH+ subpopulation is larger in orospheres in both UMSCC14B and UMSCC103 cell lines. B. Summary of marker expression in orospheres and adherent cells. *P<0.05 C. Real time PCR for the most common stem cell-related markers (OCT3/4, SOX-2, NANOG). All stem cell-related markers are remarkably more expressed on orospheres. *P<0.05. D. Growth curve of orospheres and adherent cells implanted in immunocompromised mice. In both cell lines, the orospheres show faster growth, resulting in larger tumors at the time of sacrifice. E. Examples of explanted tumors for both cell lines. Orospheres produce remarkably larger tumors. Scale bar=4mm. F. H&E staining on sections of explanted and original primary tumors. Orospheres and adherent cells recapitulate the architecture of the original tumor. Original magnification: 100X (xenograft); 400X (original primary tumor).