Fig.6. WMJ-S-001 altered p53 modification and the protein levels of p21^cip/Waf1 and survivin in HUVECs.

Cells were treated with vehicle or WMJ-S-001 at indicated concentrations for 6 h. The phosphorylation and acetylation status of p53 was then determined by immunoblotting. Complied results are shown at the bottom of the chart. Each column represents the mean ± S.E.M. of four independent experiments * p < 0.05, compared with the control group. Cells were treated with vehicle or WMJ-S-001 at indicated concentrations for 24 h, the protein levels of p21 (B) and survivin (C) were then determined by immunoblotting. Each column represents the mean ± S.E.M. of four independent experiments * p < 0.05, compared with the control group.