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. 2014 Nov 6;6(1):207–220. doi: 10.18632/oncotarget.2702

Figure 3. LMP2A down-regulated the compositional ratio of the nuclear fractions of TWIST and YB-1, and trigger their changes of subcellular localization.

Figure 3

(A) LMP2A was stably overexpressed in SGC7901 cells and transiently silenced in SNU719 cells for 48 h. Western blot analysis exibited the effect of the transfections. (B, C) The nucleus/cytoplasm ratio of TWIST (B) and YB-1 (C) decreased to 9% and 20%, respectively, after LMP2A was overexpressed in SGC7901-LMP2A cells. (D, E) Following LMP2A silenced in SNU719 siLMP2A cells, the compositional ratios of the nuclear fractions of TWIST (D) and YB-1 (E) were up-regulated to 400% and 357%, respectively. Vinculin and Lamin B1 were used as markers for the cytoplasmic and nuclear fractions respectively. (F, G) Immunofluorescent analysis showed that SGC7901-LMP2A cells have a substantial decrease in TWIST (green; F) and YB-1 (green; G) staining in both cytoplsmic and nuclear areas, and Hoechst staining was used to counter stain nuclei (blue).