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. 2014 Nov 16;6(1):243–257. doi: 10.18632/oncotarget.2801

Fig.1. CD147 regulates cell morphology and motility via coordinating Rac and Rho signalings.

Fig.1

(A) CD147 was examined in total lysates of 7721, K7721, R7721 and K7721-pcDNA3.1 cells (K7721 cells transfected with pcDNA3.1 vector as a mock control for R7721) using western blotting. (B) Wound healing assay of the 7721, K7721 and R7721 cell lines. (C) Migration assay of the 7721, K7721 and R7721 cell lines. (D) In vitro invasion assay of the 7721, K7721 and R7721 cell lines. (E) Confocal microscopy images of 7721, K7721 and R7721 cells. Red: CD147; Green: actin; Blue: DAPI. Scale bar = 20μm. (F) Rac1 activity, WAVE2 expression, and RhoA and MLC2 activities were examined in total lysates of 7721, K7721, R7721 and K7721-pcDNA3.1 cells using western blotting. (G) Src and Rac1 activities, WAVE2 expression, and RhoA and MLC2 activities were examined in total lysates of Huh-7 cells and Huh-7 cells transfected with si-147 or sncRNA using western blotting. The bars represent each sample performed in triplicate, and the error bars indicate ± SD. ** P < 0.01, * P < 0.05, by one-way ANOVA (B-D).