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. 2014 Nov 16;6(1):243–257. doi: 10.18632/oncotarget.2801

Fig.5. DOCK8 is a Rac1 GEF involved in the Src-regulated Rac/WAVE signaling pathway.

Fig.5

(A) Images (Scale bar = 500μm) and confocal microscopy images (Scale bar = 20μm) of the DOCK8 siRNA (si-D8)-induced morphological changes in 7721 cells. Green: actin; Blue: DAPI. Left panel: representative image. Right panel: quantification. (B) DOCK8 was detected using western blotting after the transfection of 7721 cells with DOCK8 siRNA. (C) The DOCK8 mRNA level was detected using real-time PCR after the transfection of 7721 cells with si-D8. (D) Effects of si-D8 on cell motility of 7721 cells. Cells were transfected with si-D8 prior to use. (E) Rac1 immunoprecipitated with DOCK8. (F) Rac1 activity, WAVE2 expression and MLC2 activity in 7721 and 7721-siD8 cells. (G) The mRNA level of DOCK8 was detected using real-time PCR after the transfection of 7721 cells with ANXA2 siRNA (si-A2). (H) DOCK8 expression was examined in total lysates of SMMC-7721, K7721, R7721 and K7721-pcDNA3.1 cells using western blotting. The bars represent each sample performed in triplicate, and the error bars indicate ± SD. ** P < 0.01, * P < 0.05, by t-test (A, C, D, G).