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. 2014 Nov 6;6(1):258–270. doi: 10.18632/oncotarget.2724

Figure 1. Resistin promotes cell migration of chondrosarcoma cells through increasing matrix metalloproteinase (MMP-2) expression.

Figure 1

The JJ012 and SW1353 cells were incubated with resistin (0.3–3 ng/ml) for 24 h, and in vitro migration was measured by (A) Transwell and (B) wound-healing assays. (C) JJ012 cells were incubated with resistin for 24 h, the protein and mRNA expressions of MMP-2 were measured by western blot (upper panel) and zymography (lower panel). The JJ012 and SW1353 cells were incubated with resistin for 24 h, and the protein and mRNA expressions of MMP-2 were measured by (D) enzyme-linked immunosorbent assay (ELISA), and (E) real-time quantitative polymerase chain reaction (RT-qPCR). (F) The JJ012 and SW1353 cells were pre-treated with an MMP-2 inhibitor or pre-transfected with MMP-2 siRNA, and the in vitro migration was measured using Transwell assays. The results are expressed as mean ± SEM. *, P < 0.05 compared with control. #, P < 0.05 compared with the resistin-treated control group.