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. 2014 Nov 6;6(1):258–270. doi: 10.18632/oncotarget.2724

Figure 4. Knockdown of resistin suppresses lung metastasis in vivo.

Figure 4

JJ012 cells stably expressing control- or resistin-shRNA were established. The protein and mRNA expressions of (A) resistin and (B) MMP-2 were examined by western blot and real-time quantitative polymerase chain reaction (RT-qPCR), respectively. (C) MiR-519d expression in the JJ012/Control-shRNA and JJ012/Resistin-shRNA cells was detected by RT-qPCR. (D) Cell migration in the JJ012/Control-shRNA and JJ012/Resistin-shRNA cells was examined by transwell assays. (E) JJ012/Luc or JJ012/shResistin-Luc cells were injected into the lateral tail vein of severe combined immune deficient mice, and the development of lung metastasis was monitored by bioluminescence imaging at the indicated time intervals. (F) Quantification of in vivo bioluminescence imaging images (photons/s of lung region). (G) After 9 weeks, the mice were sacrificed, and the lung tissues were excised and photographed. (H) Immunohistochemistry of resistin and MMP-2 protein expressions in metastatic chondrosarcoma cells from the lung. The results are expressed as mean ± SEM. *, P < 0.05 compared with the JJ012/Control-shRNA group.