Skip to main content
. 2014 Nov 16;6(1):271–287. doi: 10.18632/oncotarget.2773

Figure 7. The HAT activity of GCN5 is not required for the inhibition of XBP-1S-mediated transcription.

Figure 7

(A) IP was performed with an anti-GCN5 antibody and the cell lysates prepared from the transfected cells. IP using the normal IgG (IgG) was used as a control. The immunoprecipitated complexes and the protein inputs were analyzed by Western blotting. (B) Luciferase reporter assays were performed using a specific Luc reporter plasmid (i.e. BiP-Luc or EBV-TR-L1-Luc) and the indicated expression vectors (i.e. XBP-1S, GCN5 WT, and GCN5 mt). (C) MCF7 cells were co-transfected with a XBP-1S expression vector and an indicated GCN5 plasmid (i.e. GCN5 WT or GCN5 mt). ChIP was carried out followed by quantitative PCR to quantify the binding of XBP-1S to the BiP, CHOP, and EDEM promoters. Cells transfected with an empty vector and a XBP-1S plasmid was used as control. *P<0.05 versus controls. (D) The expression levels of XBP-1S in the transfected MCF7 cells were analyzed by immunoblotting. Actin was used as a loading control.