Skip to main content
. 2014 Nov 16;6(1):305–316. doi: 10.18632/oncotarget.2786

Figure 4. Knockdown of RBM38 enhances HIF1α expression through mRNA translation.

Figure 4

35S-metabolic labeling assay was performed with H1299 (A) or p53−/− HCT116 (B) cells. Cells were transduced with a lentivirus expressing a control luciferase (Luc) shRNA or RBM38 shRNA, selected by puromycin for 3 d, and then treated with 500 μM CoCl2 for 3 h, followed by labeling with 35S-methionine and 35S-leucine. Cell lysates were isolated and used for immunoprecipitation with anti-HIF1α (H1α67, Sigma) or non-immune mouse IgG. The samples from immunoprecipitation were separated in 8% SDS/PAGE and the protein signals were captured by autoradiography.