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. 2014 Nov 16;6(1):345–354. doi: 10.18632/oncotarget.2814

Figure 4. SULT1A1 expression predicts NSC743380-sensitivity in leukemia cell lines.

Figure 4

A) Expression of SULT1A1 in cell lysates of 18 leukemia cell lines. The cell lysates were harvested several years ago and stored them in a –80°C freezer. Cell lysates from the kidney cell line A498 were used as positive control. The results showed that U937, M07e, MV4-11, and THP-1 were positive for SULT1A1 proteins. β-Actin was used as loading control. B) Cell viability assay for six leukemia cell lines. The cells were treated with various concentrations of NSC-743380. Cell viability was determined 3 days after treatment using the MTS assay. Control cells (indicated by the 0 in the concentration) were treated with solvent (dimethyl sulfoxide), and their value was set as 1. The values represent mean ± SD of a quadruplet assay. The assay repeated at least twice with similar results.