Figure 2. DZNep elicits similar cell cycle effects as si-HOTAIR in GBM cells.

(A) Western blot analysis was used to examine the expression of LSD1, EZH2, p16, p21, Cyclin D1, Cyclin E, H3K27Me3 and H3K4Me3 in U87 and LN229 cells treated with si-HOTAIR, 2-PCPA (100 μ M) and DZNep (1 μM) for 48 h. GAPDH was used as a loading control. (B) Flow cytometry was performed to examine the G1/S arrest in U87 and LN229 after treatment with si-HOTAIR, 2-PCPA (100μM) and DZNep (1 μM) for 48 h.