Table 1. Statistics of libraries generated by TruSeq or LM-Seq.

Samples	Multiplex per lane	Total reads	Percent of non-duplicated reads*	Number of reads mapped to RefSeq	Percent of reads mapping to RefSeq	Number of genes withTPM > 1	Percent of mapped reads mapping on expected sense/antisense strand
TruSeq	12	12426491	62.4%	10929050	88%	13000	50.6%
TruSeq-Stranded mRNA Seq-1	6	28515378	33.5%	24787808	87%	12151	99.4%
TruSeq-Stranded mRNA Seq-1	94	2179618	72.3%	1910603	88%	12899	99.4%
LM-Seq-1	24	6417881	62.0%	5119315	80%	12484	97.2%
LM-Seq-2	24	8786110	53.6%	5985757	68%	12784	98.2%
LM-Seq-10 ng total RNA	24	5673905	21.1%	3770987	66%	12421	98.6%
LM-Seq-85°C 11 min	24	8391832	47.8%	5601727	68%	12752	98.2%
LM-Seq-94°C 6 min	24	8273910	27.0%	5545433	67%	12558	98.2%
LM-Seq-1 hr ligation	24	6116079	35.5%	4172809	68%	12624	98.0%
LM-Seq-3 hr ligation	24	6613601	42.7%	4381615	66%	12668	98.2%

*: As reported by FastQC version 0.11.2.