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. 2015 Apr 1;9:113. doi: 10.3389/fncel.2015.00113

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Copyright © 2015 Lin, Zhang, Wu and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FACS isolated Lgr5-positive cells self-renew to form spheres. (A,B) FACS isolated Lgr5-EGFP-positive cells generated significantly more spheres than Lgr5-EGFP-negative cells. (C) Quantification of sphere number generated from FACS isolated Lgr5-EGFP-positive and Lgr5-EGFP-negative cells over seven generations. (D) Quantification of sphere size generated from FACS isolated Lgr5-EGFP-positive and Lgr5-EGFP-negative cells over seven generations. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3 in (C,D). Scale Bars: 100 µm.