Table 2.
List of described MLC1 molecular interactors in astrocytes.
| Experimental techniques | Interaction compartment | Experimental models | Reference | |
|---|---|---|---|---|
| GlialCAM | Quantitative proteomic analysis and quantitative mass spectrometry; in situ hybridization and immunohistochemistry (IHC); immunofluorescence (IF); electron microscopy (EM) immunostaining | Membrane (COOH) | Primary rat astrocytes; HeLa cells Mlc1 (-/-) zebrafish Mlc1 (-/-) mice Human brain tissue | López-Hernández et al. (2011a,b), Capdevila-Nortes et al. (2013), Arnedo et al. (2014a,b), Barrallo-Gimeno and Estévez (2014), Sirisi et al. (2014), Dubey et al. (2015) |
| Na,K-ATPase beta1 | Yeast two-hybrid assay; Ouabain-affinity chromatography; Co-purification of His-tagged proteins and LC–MS analysis; IF; IHC | Membrane/cytosol (NH2) | Rat astrocytes and rat brain; human astrocytoma cells | Brignone et al. (2011), Brignone et al. (2014), Lanciotti et al. (2012) |
| TRPV4 | Pull-down assay; ouabain-affinity chromatography. | Membrane | Rat astrocytes; human astrocytoma cells | Lanciotti et al. (2012), Brignone et al. (2014) |
| V-ATPase | Co-purification of His-tagged proteins and LC-MS analysis; IF; IHC | Cytosol | Rat primary astrocytes; human astrocytoma cells; human brain tissue | Brignone et al. (2014) |
| DGC complex | IF; IHC; immunoprecipitation (IP); Co-purification of His-tagged proteins and co-fractionation assays; ouabain-affinity chromatography | Membrane/cytosol | Gliotic brain tissue; glioblastoma tissue and brain tissue from an MLC patient; human and rat astrocytes; rat brain extracts; human astrocytoma cells | Boor et al. (2007), Lanciotti et al. (2010, 2012), Brignone et al. (2011) |
| ZO-1 | EM immunostaining; IP | Membrane | Rat and human brain | Duarri et al. (2011) |
| Kir4.1 | IP; co-purification of His-tagged proteins and co-fractionation assays; ouabain-affinity chromatography; IF; IHC | Membrane | Human and rat astrocytes; rat brain extracts; human astrocytoma cells | Boor et al. (2007), Lanciotti et al. (2010, 2012), Brignone et al. (2011), Dubey et al. (2015) |
| Cav-1 | Pull-down assay; co-purification of his-tagged proteins; IF | Membrane (NH2; COOH) | Rat astrocytes; human astrocytoma cells | Lanciotti et al. (2010) |
| ClC2 | IHC; EM | Mlc1-null mice | Hoegg-Beiler et al. (2014), Dubey et al. (2015) |