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. 2015 Mar 25;10:2377–2389. doi: 10.2147/IJN.S76191

Figure 4.

Figure 4

Related protein and gene expressions in osteoporotic rat femurs.

Notes: (A) OPG and RANKL protein expressions in femurs of OVX rats. Effects of 12-week treatment with EV, CIT-H, CIT-M, CIT-L, CIT-SO-H, CIT-SO-M, and CIT-SO-L on protein expressions of OPG and RANKL. (B) OPG and RANKL mRNA expressions in femur of OVX rats treated with EV, CIT-H, CIT-M, CIT-L, CIT-SO-H, CIT-SO-M, and CIT-SO-L for 12 weeks. Total RNA was isolated, and reverse-transcription polymerase chain reaction was performed to determine OPG and RANKL mRNA expressions. Values are expressed as means ± standard deviation (n=8); aP<0.05 compared to OVX, bP<0.01 compared to OVX, cP<0.05 compared to CIT-L, dP<0.05 compared to CIT-M, eP<0.05 compared to CIT-H, fP<0.01 compared to CIT-H.

Abbreviations: OVX, ovariectomized; SO, suet oil; EV, estradiol valerate; CIT, circinal–icaritin; H, high dosage; M, medium dosage; L, low dosage; RANKL, receptor activator of nuclear factor-κB ligand; OPG, osteoprotegerin; mRNA, messenger ribonucleic acid; OCN, osteocalcin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.