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. 2015 Mar 17;2015:547473. doi: 10.1155/2015/547473

Figure 4.

Figure 4

Effect of the pretreatment with the PKA inhibitor H89 (2 × 10−6 M, 30 min before) on (a) β-catenin and (b) phosphorylated-GSK3β (P-GSK3β) levels measured in whole cell lysates by Western blot on rat osteoblast-like cells (rOB) treated with ghrelin (AG, 10−10 M for 1 h). β-catenin levels were normalized to β-actin levels; P-GSK3β was normalized to total GSK3β levels (Tot-GSK3β). Results are expressed as ratio of β-catenin or P-GSK3β measured in treated cells versus untreated cells. Insets: typical gels obtained from rOB cell lysates after treatment with ghrelin alone or together with H89. Data are the mean ± SEM of 5 experiments * P < 0.05 versus untreated cells; § P < 0.05 versus AG treated cells.