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. 2015 Feb 6;2(2):111–124. doi: 10.18632/oncoscience.120

Figure 6. Genistein treatment results in decreased protein synthesis in MV4-11 and HL-60 cells.

Figure 6

A) Protein synthesis was analyzed using metabolic labelling, and there is an immediate and consistent drop in the protein synthesis rates in MV4-11 cells. However, a delayed effect is observed in the HL-60 cells, where significant decrease in protein synthesis was observed after 72 h of genistein treatment. The data shown is the mean of a triplicate of experiments. * indicates p<0.05; *** indicates p <0.001. Error bars represent standard deviation. B) Western blot analysis of mTOR pathway, which is the key pathway in regulating protein synthesis and is reported to be regulated from our iTRAQ analysis. Our result shows the reduced phosphorylation of the protein 4EBP-1, where the unphosphorylated form binds to eIF4E protein and inhibits its translational activity. This results in the decreased protein synthesis, in accordance to our protein synthesis assay.