Table 1. Guide to Supplementary File 1.

Column	Column Header	Explanation
1	Sample_ID	Sample identifier coding organ, sex, age, and replicate #
2	Libray_Processing_Order	The order in which an RNA sample or library was processed
3	RNA_Sample_ID	Sample identifier specifying the original serial sample ID
4	Flowcell	The flowcell number on which the sample was sequenced
5	Lane	The lane number on which the sample was sequenced
6	ERCC_Mix	Either ERCC Mix1 or Mix2 was added to the RNA sample
7	BarCode	The multiplexing barcode for the RNA sample
8	RNA_A260.A280_Ratio	A260/A280 ratio indicating RNA purity
9	RNA_RIN	RNA Integrity Number
10	Library_Con_ng.ul	Library concentration in ng μl−1
11	Organ	Full name of the organ from which the RNA was isoloated
12	Organ_Abbr_2chars	Two-character abbreviation for an organ
13	Organ_Abbr_3chars	Three-character abbreviation for an organ
14	Age_Week	Age (weeks) of the rat
15	Sex	Sex of the rat
16	Replicate	Replicate #
17	Sample_Name_Alt	Alternate name with two-character code for organ name
18	Genome_Ratio	The ratio of reads mapped to genome
19	Aceview_Ratio	The ratio of reads mapped to the AceView database
20	ERCC_Ratio	The ratio of reads mapped to the ERCCs
21	rRNA_Ratio	The ratio of reads mapped to rRNA
22	Total_Reads	The number of total reads collected on an RNA sample