Figure 2.

Female DNAm at TSSs shows a continuous range from subject to escape. (A) The average level of female DNAm in PB ranked from highest to lowest. XCI status of each TSS is denoted by colour (red: subject, purple: variable escape, grey: uncallable and green: escape) with error bars representing 1 SD. (B) Linear regression of AI and average female PB DNAm (red: subject, and green: escape). (C) Linear regression of average female DNAm on PB and BU. (D) Variable escape TSSs rarely disagreed in XCI status within a twin pair. A comparison of the DNAm within twin pairs and coloured according to WB twin XCI status revealed a high level of agreement in average twin TSS DNAm. Most TSSs had the same XCI status within a twin pair [subject in both: solid red circle, uncallable in both (solid grey circle) or escape in both (solid green circle)] or were uncallable in one twin but subject (open red circle) or escaped (open green circle) in the other twin. A limited number of variable escape TSSs were subject in one twin but escaped from XCI in the other (solid black circle). R² and P-values for each linear regression are given on their respective graphs.