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Neural tube defects in Shmt1-deficient embryos at gestational day 11.5 (E11.5). (A) Representative unaffected embryo. (B) Shmt1+/− embryo from a dam fed a folate-deficient diet supplemented with thymidine diet exhibited failure of rostral neural tube closure beyond the hindbrain boundary. Scale bars indicate 1 mm. Arrows indicate extent of lesions.
