. 2014 Sep 25;3:e03772. doi: 10.7554/eLife.03772

Table 1.

Enzymatic Parameters of different length IGF1R proteins^‡^§

DOI: http://dx.doi.org/10.7554/eLife.03772.021

Table 1—source data 1. Representative curves of steady-state kinetic analyses for each IGF1R protein characterized.

Each data point was performed in duplicate and is shown separately.

DOI: http://dx.doi.org/10.7554/eLife.03772.022

DOI: 10.7554/eLife.03772.022

Table 1—source data 2. Enzyme behavior is linear with respect to time.

Product/Enzyme plotted vs time (minutes) for each IGF1R protein investigated.

DOI: http://dx.doi.org/10.7554/eLife.03772.023

DOI: 10.7554/eLife.03772.023

Table 1—source data 3. Enzyme behavior is linear with respect to enzyme concentration.

Velocity (nM of product/min) plotted vs enzyme concentration (nM) for each IGF1R protein investigated.

DOI: http://dx.doi.org/10.7554/eLife.03772.024

DOI: 10.7554/eLife.03772.024

	enzyme pY	K_m^app ATP (μM)	K_m^app Peptide (μM)	k_cat (min⁻¹)	k_cat/Km^app_(ATP)
IGF1R-fl + IGF1	+	82 ± 11	48 ± 8	459 ± 17	5.6
IGF1R-fl + IGF1	−	356 ± 61	68 ± 19	44 ± 3	0.1
IGF1R-fl	+	89 ± 18	91 ± 8	749 ± 22	8.5
IGF1R-fl	−	172 ± 35	66 ± 17	62 ± 5	0.4
IGF1R-icd	+	79 ± 9	110 ± 12	385 ± 13	4.9
IGF1R-icd	−	>1000^*	>1000^*	N.D.^†	0.0013
IGF1R-jmk	+	68 ± 12	52 ± 5	363 ± 20	6.9
IGF1R-jmk	−	>1000^*	>1000^*	N.D. ^†	0.0029
IGF1R-kin	+	115 ± 24	114 ± 26	98 ± 6	0.85
IGF1R-kin	−	>2000^*	>1250^*	N.D. ^†	0.0019

The absolute value of Km could not be determined but is greater than value stated.

^†

k_cat value could not be measured.

^‡

Enzymatic values (±s.d.) calculated from duplicate experiments are shown.

^§