Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 21;3:e03881. doi: 10.7554/eLife.03881

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014, van Bragt et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 3. — (A) Schematic representation of the Runx1 conditional knockout allele in which its exon 4 is flanked by loxP sites, as well as the R26Y conditional Cre-reporter. STOP: transcriptional stopper cassette. Subsequent breeding with MMTV-Cre resulted in mice in which selected subsets of MECs express YFP and lack expression of functional RUNX1. (B) FACS gating strategy for detecting lin⁻YFP⁺ (lin: lineage markers) MECs, as well as YFP⁺ lin⁻CD24⁺CD29^lo luminal (Lu), and lin⁻CD24⁺CD29^hi basal (Ba) MECs in MMTV-Cre;R26Y females. Str: stromal cells. (C) qRT-PCR analysis confirming the loss of Runx1 expression in YFP⁺ MECs sorted from MMTV-Cre;Runx1^L/L;R26Y females (L/L). (D) FACS analysis showing the reduced lin⁻YFP⁺ MEC population (left plots), as well as the reduced lin⁻YFP⁺ luminal population (right plots), in MMTV-Cre;Runx1^L/L;R26Y female compared to those in MMTV-Cre;Runx1^+/+;R26Y control female. (E–F) The percentages of lin⁻YFP⁺ MEC population (E), as well as the ratios of luminal/basal subpopulations among the lin⁻YFP⁺ gate (F), are significantly reduced in MMTV-Cre;Runx1^L/L;R26Y females (n = 9) (L/L) compared to those in MMTV-Cre;Runx1^+/+;R26Y control females (n = 10) (+/+). p values: *: p ≤ 0.05; error bars represent mean ± S.E.M.

DOI: http://dx.doi.org/10.7554/eLife.03881.006

Figure 3—figure supplement 1. — (A) Schematic representation of the Runx1 conditional knockout allele in which its exon 4 is flanked by loxP sites, as well as the R26Y conditional Cre-reporter. STOP: transcriptional stopper cassette. Subsequent breeding with MMTV-Cre resulted in mice in which selected subsets of MECs express YFP and lack expression of functional RUNX1. (B) FACS gating strategy for detecting lin⁻YFP⁺ (lin: lineage markers) MECs, as well as YFP⁺ lin⁻CD24⁺CD29^lo luminal (Lu), and lin⁻CD24⁺CD29^hi basal (Ba) MECs in MMTV-Cre;R26Y females. Str: stromal cells. (C) qRT-PCR analysis confirming the loss of Runx1 expression in YFP⁺ MECs sorted from MMTV-Cre;Runx1^L/L;R26Y females (L/L). (D) FACS analysis showing the reduced lin⁻YFP⁺ MEC population (left plots), as well as the reduced lin⁻YFP⁺ luminal population (right plots), in MMTV-Cre;Runx1^L/L;R26Y female compared to those in MMTV-Cre;Runx1^+/+;R26Y control female. (E–F) The percentages of lin⁻YFP⁺ MEC population (E), as well as the ratios of luminal/basal subpopulations among the lin⁻YFP⁺ gate (F), are significantly reduced in MMTV-Cre;Runx1^L/L;R26Y females (n = 9) (L/L) compared to those in MMTV-Cre;Runx1^+/+;R26Y control females (n = 10) (+/+). p values: *: p ≤ 0.05; error bars represent mean ± S.E.M.

DOI: http://dx.doi.org/10.7554/eLife.03881.006