Table 1.

Primers used to amplify rat bone and muscle markers

Gene primer sequences 5′ to 3′	Td	Amplicon	Accession number
Bone markers
Rat Osteocalcin		285 bp	M23637
F gcc cag cga ctc tga gtc tg	67.2
R cca atg tgg tcc gct agc tc	67.2
Rat Bone Sialoprotein		264 bp	DQ213013
F gta ccg gcc acg cta ctt tc	66.5
R cca gcg tca gct gtg gtc tc	68.0
Rat Alkaline phosphatase		260 bp	J03572
F ccc tgc aac cat cat gga ac	67.4
R gaa ggg ttg gcg agt ctc tg	66.2
Rat Osteopontin		289 bp	M14656
F gcc agc ctg gaa cat cag ag	67.1
R aag ctc agg gcc caa aac ac	68.2
Rat Collagen I alpha 1		275 bp	XM_213440
F ggg gtg gga agg aat ttc tg	67.3
R tag gga gca gca gca aga gg	67.2
Rat Cbfa1		221 bp	AB025797
F agt ggc cag gtt caa cga tc	66.3
R tgc gcc cta aat cac tga gg	67.8
Muscle markers
Rat Myosin 1		250 bp	X68199
F aag cag cta gta agg gtg act ttc	65.2
R ctc act taa ggg aca gcg act tc	66.7
Rat Actin alpha		329 bp	NM_019183
F tcc cag cac cat gaa gat taa g	66.1
R agg ttg caa gtc ctg gtc tg	64.0
Housekeeping gene
Rat Gapdh		188 bp	AF106860
F aag ggc atc ctg ggc tac ac	68.0
R gag gcc atg tag gcc atg ag	66.9