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. 2015 Apr 1;10(4):e0120751. doi: 10.1371/journal.pone.0120751

Fig 2. Tandem cores form VLPs when produced in E. coli.

Fig 2

a) Western blot showing expression in induced (+) and uninduced (-) E. coli of homo- and hetero- tandem core with either 5 (GGS5) or 7 (GGS7) copies of the GGS sequence in the flexible linker between core 1 and core 2. b) Coomassie-stained gel of sucrose gradient fractions of CoHo (E. coli codon-optimised homo-tandem core with GGS7) produced in E. coli. The major band (fraction 2) reacted with anti-HBcAg antibody in western blot analysis. c) Electron micrographs of monomeric (HBcΔ149), codon-optimised homo-tandem (CoHo) and hetero-tandem (CoHe) core particles produced in E. coli and purified by sucrose gradient. Scale bar 100 nm. Arrows indicate smaller (T = 3) particles.