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. 2015 Apr 1;10(4):e0122649. doi: 10.1371/journal.pone.0122649

Fig 4. Southern blot analyses of BbSQEs-I and -II using genomic DNA.

Fig 4

The cDNA fragments of 325-bp BbSQE-I, or 1477-bp BbSQE-II were labeled with digoxigenin, and used as probes for detecting corresponding genomic fragments. Upper panels show the Southern blots. After stripping probe of BbSQE-I, the same membrane was re-hybridized with that of BbSQE-II. The representative drawings at the bottom show positions of probe regions (arrows), as well as PstI and EcoRI sites (vertical bars with a letter) in the ORF (rectangles). Scale bar for cDNA length is indicated at the bottom.