Individual species of DAG were analyzed in the liver, heart and soleus muscle of rats from all groups; in the case of the VR groups the rats were selected on the basis of the intensity of their activity (5–8 km/day). From these results, the percentage proportions of saturated fatty acids (SFA expressed as 18:0 + 16:0), monounsaturated fatty acids (MUFA, 18:1) and polyunsaturated fatty acids (PUFA, 18:2) in tissue DAG were calculated. The data are expressed as mean ± SEM, * significant effect of voluntary running within the dietary groups (CD+VR against CD; HSD+VR against HSD); Two-way ANOVA results: ‡ significant effect of VR, † significant effect of HSD, # significant interaction between VR and HSD; P < 0.05. Abbreviations: DAG, diacylglycerol; HHTg, hereditary hypertriacylglycerolemic rats; HSD, high-sucrose diet; VR, voluntary running.