Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nephron Exp Nephrol. 2014 Nov 19;128(0):80–88. doi: 10.1159/000368411

Fig. 4.

Fig. 4

wmISH demonstrates W nt5a expression in the mouse IM, mesonephros and metanephric kidney. wmISH of Ret gene expression (a–d) is used to identify IM and UB location at E10.5 (a), E11.5 (b), E13.5 (c) and E15.5 (d). Red dashed lines outline the IM tissues. At E10.5, while Ret is expressed in the ND and at the site of UB outgrowth (a), Wnt5a is diffusely expressed at a lower level in the entire IM region with higher expression caudally (e and g). f, h At E11.5, Wnt5a expression level increases significantly in the gonad and mesonephric kidney, but not in the metanephric region where the UB initiates. At E13.5 (i) and E15.5 (j), Wnt5a is expressed in the metanephric kidney, with a pattern similar to Ret expression. Bar = 200 μm.