Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Exp Neurol. 2014 Dec 24;265:8–21. doi: 10.1016/j.expneurol.2014.12.015

Fig. 8.

Fig. 8

Microglia activation in lesion areas with reduced AQP4 expression. A: Reduction of AQP4 expression in areas of human anti-AQP4 IgG deposition is shown by immunohistochemistry using two different commercial polyclonal anti-AQP4 ABs (upper panel: sc-20812, Santa Cruz, USA. Lower panel: S-A5971, Sigma-Aldrich, USA). Dashed lines were drawn to better illustrate the regions of pronounced human IgG deposition (NMO1, red). Both commercial ABs showed a similar reduction of AQP4 immunoreactivity in lesion areas (scale bar: 200 μm). B: Iba-1 expression representing microglial cells was increased largely with hypertrophy and proliferation in lesion areas with identified deposition of human IgG as shown in other sections when compared to control IgG treated animals. Higher magnification (lower panel) showed hypertrophic and ramified microglia in the white matter of the spinal cord (scale bar: upper panel in b: 100 μm, lower panel: 50 μm).