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. 2014 Nov 21;125(1):117–128. doi: 10.1172/JCI73935

Figure 6. mTORC2 is required for ENaC-mediated Na+ current: direct measurement by patch clamp.

Figure 6

The effect of mTOR on ENaC and ROMK activity was assessed by whole-cell patch clamp of microdissected rat kidney CCD. After isolation, tubules were preincubated for 30 minutes with or without 1 μM PP242. (A) Currents through ENaC (INa) were measured as the difference in current at Vm –100 mV with and without 10–5 M amiloride. n = 17 (PP242) and 24 (control) cells from 4 animals. (B) Currents through ROMK (ISK) were measured as the difference in current at Vm –100 mV with and without 10–7 M TPNQ. n = 20 (PP242) and 23 (control) cells from 4 animals. Data represent mean ± SEM; differences were determined by repeated-measures 1-way ANOVA. *P < 0.05 vs. vehicle.