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. 2014 Dec 8;125(1):337–349. doi: 10.1172/JCI77172

Figure 3. Effect of decreased arcuate nucleus glucokinase activity on food and glucose intake.

Figure 3

(A) Glucokinase (GK) activity in Hep G2 cell lysates after transfection with either rAAV-GFP or rAAV-ASGK (n = 6). (B) Glucokinase activity in arcuate nucleus, VMN, and PVN of male Wistar rats following intra-arcuate injection of either rAAV-GFP (iARC-GFP) or rAAV-ASGK (iARC-ASGK). (C) Cumulative food intake and (D) weight changes in iARC-GFP (black diamonds) and iARC-ASGK (white diamonds) rats after recovery from surgery (n = 11). (E) 2% w/v glucose solution intake, (F) food intake, and (G) energy intake in iARC-GFP (black diamonds) and iARC-ASGK (white diamonds) rats during a 24-hour feeding study, with ad libitum access to 2% w/v glucose solution and normal chow intake (n = 11). (H) 10% w/v glucose, (I) food intake with normal chow, (J) total energy intake, and (K) weight changes in iARC-GFP (black diamonds) and iARC-ASGK (white diamonds) rats, with ad libitum access to normal chow diet and 10% w/v glucose given 4 weeks after rAAV microinjection (n = 11). Data presented as mean ± SEM *P < 0.05, ***P < 0.001 versus corresponding control values.