Skip to main content
. 2014 Dec 8;125(1):316–323. doi: 10.1172/JCI76802

Figure 6. Activity of SENP2 in patient-derived PBMCs.

Figure 6

SENP2 enzymes derived from PBMCs of a patient and a healthy donor were purified by affinity chromatography and incubated with GST-RanGAP-SUMO1. Analysis was done by Western blotting and immunodetection using GST mAb. Desumoylation was detected for both preparations, indicating an active SENP2 enzyme in patients and healthy controls. (A) SENP2 isolation from PBMCs. Detection was done by Western blot using anti-SENP2. Lanes 1–3: healthy donor; lanes 4–6: patient. Lanes 1 and 4: lysate; lanes 2 and 5: flow through; lanes 3 and 6: eluate. (B) Test for SENP2 activity. Detection was done by Western blot using anti-GST. Lane 1: GST-RanGAP as control; lane 2: GST-RanGAP plus patient-derived SENP2; lane 3: GST-RanGAP plus SENP2 from healthy donors. The upper band represents GST-RanGAP-SUMO1, the lower GST-RanGAP.