Figure 5. Half dosage of mGluR1 restores motor performances of Afg3l2^+/– mice.

(A) Beam-walking test performed on Afg3l2^+/– Grm1^+/crv4 mice and their littermates at 8 and 12 months of age. Data represent the mean ± SD of 4 independent tests; n = 13–15. *P < 0.05 and **P < 0.001 by Student’s t test. (B) Cryostat-cut sections of cerebellum from Afg3l2^+/– Grm1^+/crv4 mice and their littermates at 8 months of age stained with anti-calbindin Ab. Scale bar: 100 μm.(C) Semithin sections of cerebellum from Afg3l2^+/– Grm1^+/crv4 mice and their littermates at 8 months of age stained with toluidine blue. Arrows indicate dark PCs. (D) Quantification of healthy and dark PCs in 8-month-old Afg3l2^+/– Grm1^+/crv4 mice. Data represent the mean ± SD; n = 4. P < 0.001 by Student’s t test for both healthy (*) and dark (§) PCs. (E) WB analysis of cerebellar extracts from of Afg3l2^+/– Grm1^+/crv4 mice and their littermates using anti-spectrin Ab. A cerebellar extract treated with 2 mM CaCl₂ and 1 U calpain was used as a positive control. Actin was used to verify equal loading.