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. 2014 Nov 21;125(1):141–156. doi: 10.1172/JCI75852

Figure 1. miR-21 upregulation in Alport nephropathy precedes histological changes in the kidney.

Figure 1

(A) Schema showing the sequence and configuration of human (hsa) pre–miR-21, the processed mature miR-21. The domain that binds to 3′ UTR regions of translated mRNA by sequence complementarity is highlighted. (B) Quantitative reverse-transcriptase PCR (qRT-PCR) for miR-21 copy number in tubules and glomeruli of Col4a3–/– kidneys purified by laser-capture microdissection. (C) qPCR levels for miR-21 in whole kidney from Col4a3–/– mice compared with heterozygotes. (D) PAS-stained images of kidney cortex from Col4a3–/– mice or heterozygotes showing essentially normal histology at 3 weeks and rare segmental sclerosis of glomeruli (arrow) at 5 weeks. The tubules appear normal. Scale bars: 50 μm. n = 3–6/group. *P < 0.05, 1-way ANOVA or Mann-Whitney U test)