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. 2014 Dec 15;125(1):386–402. doi: 10.1172/JCI76738

Figure 4. Intestine-specific Fxr disruption promotes mitochondrial biogenesis in the intestine.

Figure 4

(A) Serum ceramide levels in vehicle- and tempol-treated Fxrfl/fl and FxrΔIE mice after 16 weeks of HFD feeding. n = 5 mice per group. (B) Ileal ceramide levels in vehicle- and tempol-treated Fxrfl/fl and FxrΔIE mice after 16 weeks of HFD feeding. n = 5 mice per group. (A and B) Data are presented as the mean ± SD. One-way ANOVA with Tukey’s correction. *P < 0.05 and ‡P < 0.01 compared with vehicle-treated mice of the same genotype. C, side chain carbon number. (C) mRNA levels of mitochondrial oxidative phosphorylation–related enzymes in ileal mucosa from Fxrfl/fl and FxrΔIE mice after 14 weeks of HFD feeding. Expression was normalized to 18S RNA. n = 5 mice per group. mt, mitochondrial. (D) Measurement of state 3 respiration for complex I– and complex II–dependent respiration from the ileal mucosa of Fxrfl/fl and FxrΔIE mice after 14 weeks of HFD feeding. n = 4 mice per group. (C and D) Data are presented as the mean ± SD. *P < 0.05 and ‡P < 0.01 (2-tailed Student’s t test) compared with Fxrfl/fl mice.