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. 2014 Dec 15;125(1):437–447. doi: 10.1172/JCI78794

Figure 6. Polymorphisms between amino acids 526 and 569 (β-sheet) of resistant E1E2 clone 1a142 confer resistance to multiple broadly neutralizing mAbs.

Figure 6

Neutralization by the indicated mAb of HCVpp with E1E2 chimeras between resistant clone 1a142 and sensitive clone 1b09 (1b09/1a142-β and 1b09/1a142-F [F442I]), E1E2 with F442I introduced by site-directed mutagenesis (1b09/F442I), and with resistant E1E2 clone 1a142 (1a142 wild type), which carries F442I naturally. Chimeras with the front layer (1a142-F, amino acids 412–444) from resistant clone 1a142 are marked with cyan. Chimeras with β6-β8 (1a142-β, amino acids 526–569) from resistant clone 1a142 are marked in red. Orange indicates clones with F442I polymorphisms. Dashed lines indicate 50% neutralization. Error bars indicate SD between duplicate wells.