Fig 1. αII-Spectrin is localized in different invadosomes.

(A) αII-Spectrin localization in PMA-treated HMEC-1 cells and SrcY527F-MEF cells. Starved HMEC-1 cells were treated for 1hr with PMA (50 ng/ml) in order to induce characteristic invadosome rings (zoom red square). Then, endogenous cortactin and αII-spectrin were stained and αII-spectrin relocalization after invadosome induction visualized. The presence of endogenous αII-spectrin was also seen in invadosome rings from SrcY527-MEF cells. (B) 3D localization of αII-spectrin in invadosomes. Confocal Z-stacks were obtained in SrcY527F-MEF cells stained for cortactin or F-actin. αII-Spectrin dashed lines represent the projected areas (Y section is projected on the right and X section on the bottom of the image), showing preferential αII-spectrin localization at the basis of the invadosome ring. Scale bar: 5μm.