Fig 3. Changes in vertical tap root length from 0–8 DAS of plants expressing various AlaATs in both Col-0 and alaat1;2 backgrounds when grown in non-limiting N conditions.

Transgenic and control plants were sown on modified 0.5 MS with 2 mM NO₃ ^- as the sole N source. The vertical mean growth of tap roots between 0–5 and 5–8 DAS was measured (cm) in controls and transgenics at these time points and was compared using two-way ANOVA (α = 0.05, P < 0.05). At each time point for each line in each background n = 33–36. a-e) Transgenics are in an alaat1;2 background; f-j) transgenics are in a Col-0 background. * indicates significance in relation to control plants grown during the same time frame on the same plates. Error bars indicate SEM.