Fig 8. Uptake of ³H-leucine and ¹⁴C-alanine by AlaAT-expressing protoplasts at 2 hr time point.

Mesophyll protoplast cells from plant lines over-expressing AlaAT in Col-0 grown in soilless-medium under short days (12 hrs light/12 hrs dark). Uptake of label, a) ³H-leucine or b) ¹⁴C-alanine, was monitored at the 2 hr time point. Protoplasts from Col-0 and alaat1;2 lines were prepared and used as controls. Two-way ANOVA (α = 0.05, P > 0.05) was used to analyze the data, with a Bonferroni post-test to compare all transgenic lines to Col-0. * indicates significance in relation to Col-0. Error bars indicate SEM.