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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Blood. 2002 Jan 15;99(2):499–506. doi: 10.1182/blood.v99.2.499

Figure 4. Immunocomplexes between cdk4 and cyclin D2 were detected only in the presence of anti–TGF-β antibody.

Figure 4

After starvation in methionine-free DMEM for 4 hours, CD34+ progenitors were metabolically labeled for 18 hours with 35S-methionine/cysteine in the presence or absence of soluble TGF-β1 or anti–TGF-β antibody. Cell lysates were first immunoprecipitated with agarose-coated polyclonal CDK4 antibody. Subsequent serial immunoprecipitation was then carried out further using agarose-coated polyclonal cyclin D2 antibody. Samples were run on a 10% SDS-PAGE gel, dried, and exposed to autoradiogram film.