Skip to main content
. 2015 Apr 1;10(4):e0121930. doi: 10.1371/journal.pone.0121930

Fig 2. FACS analysis of CD11b-positive peripheral blood mouse monocytes.

Fig 2

FACS analysis was performed on peripheral blood mouse monocytes isolated from young (14 days; A; n = 13) or adult (6 month, B; n = 9) C57BL/6N mice following CD11b pluriBead (Pluriselect) cell separation. CD11b-positive cells were evaluated immediately following isolation (A&B; n = 9) or following 24 h incubation (C-I; n = 9). Cells were evaluated for surface marker expression of CD11b and CD45 (E&F, green) as well as their ability to phagocyte 1 μm fluorescent microspheres (G; n = 10), or FITC-Dextran (H; n = 11) or FITC-Aβ1–42 (I; n = 13). IgG controls (IgG2a and IgG1) were used as a negative control (D).