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. 2015 Apr;135(4):998–1007.e6. doi: 10.1016/j.jaci.2014.08.030

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Fig 2 — INO80 complex knockdown impairs immunoglobulin CSR in CH12-F3 cells. Quantification of CSR to IgA (on day 2 after activation) in CH12-F3 cells expressing indicated shRNA (black and grey indicate different shRNA) (A). P values from a paired Student t test: *(P < .05); **(P < .01); and ***(P < .001) are indicated. Number of viable CH12-F3 cells at the end of CSR cultures (2 days) (B) described in A. Statistically significant P value from an unpaired Student t test is indicated as *(P < .05). Immunoblot analysis of INO80, AID, YY1, and GAPDH (on day 2 after stimulation) in whole-cell extracts of CH12-F3 cells expressing indicated shRNA (C). Relative levels of specific shRNA target transcripts in CH12 cells transduced with the indicated shRNA (D). Results are presented relative to empty vector and normalized against levels of GAPDH mRNA. Real-time quantitative PCR analysis of AID transcripts (E) and S alpha GLT (F) in RNA from CH12-F3 cells expressing indicated shRNA. Results are presented relative to controls and were normalized against levels of GAPDH mRNA.