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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Nat Methods. 2015 Feb 16;12(4):319–322. doi: 10.1038/nmeth.3292

Figure 3.

Figure 3

Volume stitching results on C-PLT prepared fly brain. (a) FIB-SEM images of two sequential 20 µm ultrathick sections (#34 and #35) showing quality of cut surfaces (scale bar 10 µm). (b) Volume stitching results over an 8×12 µm cropped region. The dashed red line on the 3D volume shows the stitch plane. Images with colored borders correspond to cut planes through this 3D stitched volume (Supplementary Video 10) (scale bar 1 µm). (c-d) Side-by-side comparison of aligned hot knife cut edges before (c) and after (d) algorithmic flattening and stitching (scale bars 1 µm). These are slices taken out of a 4×4×4 µm volume which was also traced (e-f). Supplementary Video 12 contains side-by-side movies of all slices of this same volume both before and after stitching as well as after volume tracing. (g) Graphical depiction of a 9×10 µm cropped region which has been split open along the stitch plane. All processes in the densely packed central region were traced across the gap (Supplementary Video 11).