Adult rats received saline or AMI (5mg/kg, i.p.) for 14, 17, 24 or 42 days. Each hemisphere of the region of interest was dissected on ice and analyzed independently with ELISA for BDNF. Levels of BDNF are depicted for each of the following regions: Hippocampus (A), substantia nigra (B), striatum (C), frontal cortex (D), anterior cingulate (E) and piriform cortex (F). Data from AMI-treated rats are depicted in grey and data from saline-treated animals are depicted in black. Values are expressed as a percentage of absolute values in the saline control group. Levels of significance determined by Dunnett’s multiple comparison post-hoc analysis comparing all time points to the baseline control value. Data represent mean +/-SEM and are combined from two separate experiments. Abbreviations: AMI: amitriptyline, TXT: treatment, BDNF: brain derived neurotrophic factor *p<0.05, **p<0.01